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首页> 外文OA文献 >Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities
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Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities

机译：枯草芽孢杆菌BBG100过量生产霉菌素增强了生物体的拮抗和生物防治活性

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摘要

A Bacillus subtilis derivative was obtained from strain ATCC 6633 by replacement of the native promoter of the mycosubtilin operon by a constitutive promoter originating from the replication gene repU of the Staphylococcus aureus plasmid pUB110. The recombinant strain, designated BBG100, produced up to 15-fold more mycosubtilin than the wild type produced. The overproducing phenotype was related to enhancement of the antagonistic activities against several yeasts and pathogenic fungi. Hemolytic activities were also clearly increased in the modified strain. Mass spectrometry analyses of enriched mycosubtilin extracts showed similar patterns of lipopeptides for BBG100 and the wild type. Interestingly, these analyses also revealed a new form of mycosubtilin which was more easily detected in the BBG100 sample. When tested for its biocontrol potential, wild-type strain ATCC 6633 was almost ineffective for reducing a Pythium infection of tomato seedlings. However, treatment of seeds with the BBG100 overproducing strain resulted in a marked increase in the germination rate of seeds. This protective effect afforded by mycosubtilin overproduction was also visualized by the significantly greater fresh weight of emerging seedlings treated with BBG100 compared to controls or seedlings inoculated with the wild-type strain.
机译：通过用源自金黄色葡萄球菌质粒pUB110的复制基因repU的组成型启动子代替分支杆菌素操纵子的天然启动子，从菌株ATCC 6633获得枯草芽孢杆菌衍生物。命名为BBG100的重组菌株产生的霉菌枯草杆菌素比野生型多出15倍。过量产生的表型与对几种酵母和致病真菌的拮抗活性增强有关。修饰菌株中的溶血活性也明显增加。富集的霉菌枯草杆菌提取物的质谱分析表明，BBG100和野生型脂肽的模式相似。有趣的是，这些分析还揭示了一种新形式的霉菌枯草杆菌素，在BBG100样品中更容易检测到。当测试其生物防治潜能时，野生型菌株ATCC 6633对减少番茄幼苗的腐霉菌感染几乎无效。但是，用BBG100高产菌株处理种子会导致种子发芽率显着提高。与对照或接种野生型菌株的幼苗相比，用BBG100处理的出苗幼苗的鲜重明显更大，也可以看出霉菌枯草素过度生产所提供的这种保护作用。

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